The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site. Incubation Conditions: Buffer J. They recognize a specific DNA sequence, usually short (3 to 8 bp ), and cut it, producing either blunt or overhung ends, either at or nearby the recognition site . Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. Cut: Cutting site and DNA products of the cut. Cut Site: CCC GGG GGG CCC. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. The recognition sequence and the cut site usually match, but sometimes the cut site can be dozens of nucleotides away from the recognition site. Isoschizomers: TspMI, XmaCI, XmaI. Isoschizomers and neoschizomers: An isoschizomer is an enzyme that … Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol. Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Thermo Scientific FastDigest SmaI restriction enzyme recognizes CCC^GGG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. The classical restriction enzymes cut up, and hence render harmless, any unknown (non-cellular) DNA that enters a bacterial cell as a result of a viral infection. Ten enzymes were investigated: seven enzymes with a single cut site (EcoRI, KpnI, NdeI, NotI, NruI, SmaI, XbaI), two enzymes with two cut sites (BstZ17I, EagI), and one enzyme with no cut site (ClaI). Some enzymes such as SmaI cut the restriction site exactly in the middle on both strands producing cut DNA products with blunt ends. Most restriction enzymes cut their corresponding restriction sites in a staggered fashion leaving single-stranded overhangs. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. Restriction enzymes: Restriction endonucleases are used to enrich methylated from unmethylated DNA. Cut: Displays the cut site and pattern and products of the cut. 25°C. Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Note: XmaI is a neoschizomer of SmaI. In addition, we observe a decrease in alignment upon further digestion and subsequent shortening of the DNA. Isoschizomers enzymes HpaII-MspI and SmaI-XmaI recognize CCGG and CCCGGG, respectively, but HpaII and SmaI lack activity when a methyl group is present in their recognition site [61]. Source: Serratia marcescens. (SmaI exhibits 25–50% activity at 37°C.) 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